1J19
Crystal structure of the radxin FERM domain complexed with the ICAM-2 cytoplasmic peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 100.440, 100.440, 99.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 * - 2.400 |
R-factor | 0.229 * |
Rwork | 0.228 |
R-free | 0.24000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gc7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.350 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.071 * | 0.137 |
Total number of observations | 599708 * | |
Number of reflections | 23153 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 | 4 * | PEG6000, MES, NaCl, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein/peptide solution | 0.27 (mM) | |
2 | 1 | 2 | MES/Na | 50 (mM) | pH6.0 |
3 | 1 | 2 | PEG6000 | 2 (%) | |
4 | 1 | 2 | 130 (mM) | ||
5 | 1 | 2 | dithiothreitol | 0.4 (mM) |