1J11
beta-amylase from Bacillus cereus var. mycoides in complex with alpha-EPG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1997-04-18 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.900, 112.900, 146.200 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.200 |
| R-free | 0.24600 |
| Starting model (for MR) | 5bca |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.023 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.400 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.288 |
| Total number of observations | 365923 * | |
| Number of reflections | 143718 | 10261 * |
| Completeness [%] | 66.4 | 38.1 |
| Redundancy | 2.6 * | 1.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Oyama, T., (1998) Protein Pept.Lett., 5, 349. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium chloride | 50 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 6-8 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium chloride | 50 (mM) |
