1J0R

Crystal structure of the replication termination protein mutant C110S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-07
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	76.880, 50.940, 69.650
Unit cell angles	90.00, 91.17, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.233
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bm9
RMSD bond length	0.007
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.045 *	0.471 *
Total number of observations	54110 *
Number of reflections	9541
Completeness [%]	88.0	92.2
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	22 *	22% PEG 2000, 150mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)	pH6.7
3	1	reservoir	PEG2000	24 (%)
4	1	reservoir	MES	150 (mM)	pH6.0