1J0R
Crystal structure of the replication termination protein mutant C110S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.880, 50.940, 69.650 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.233 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bm9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.045 * | 0.471 * |
| Total number of observations | 54110 * | |
| Number of reflections | 9541 | |
| Completeness [%] | 88.0 | 92.2 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 * | 22 * | 22% PEG 2000, 150mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | pH6.7 |
| 3 | 1 | reservoir | PEG2000 | 24 (%) | |
| 4 | 1 | reservoir | MES | 150 (mM) | pH6.0 |
