1J0P
Three dimensional Structure of the Y43L mutant of Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.868, 67.309, 34.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 0.910 |
R-factor | 0.1015 |
Rwork | 0.099 |
R-free | 0.13600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2cdv |
RMSD bond length | 0.028 |
RMSD bond angle | 0.053 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.343 | 0.960 |
High resolution limit [Å] | 0.910 * | 0.910 |
Rmerge | 0.044 | 0.221 |
Number of reflections | 82055 | |
Completeness [%] | 95.7 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | 10 * | ethanol, pH 7.4, SMALL TUBES, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 12.5 (mM) | pH7.4 |
3 | 1 | drop | ethanol | 50 (%(v/v)) | |
4 | 1 | reservoir | Tris-HCl | 10 (mM) | pH7.4 |
5 | 1 | reservoir | ethanol | 60 (%(v/v)) |