1J0N
Crystal Structure of Bacillus sp. GL1 Xanthan Lyase that Acts on Side Chains of Xanthan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 2002-06-14 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.357, 91.305, 159.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.400 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.24200 * |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.310 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASES |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.300 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.091 * | 0.376 * |
Total number of observations | 55703 * | |
Number of reflections | 28677 | 2308 * |
Completeness [%] | 89.9 | 53.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PEG 4000, ammonium formate, sodium bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.18 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 23 (%) | |
3 | 1 | reservoir | ammonium formate | 0.2 (M) | |
4 | 1 | reservoir | sodium bicine | 0.1 (M) | pH9.0 |