1J0M

Crystal Structure of Bacillus sp. GL1 Xanthan Lyase that Acts on Side Chains of Xanthan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	2002-06-13
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	54.323, 91.446, 160.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.175
Rwork	0.175
R-free	0.24000
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.290 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.600	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.084 *	0.368 *
Total number of observations	112089 *
Number of reflections	34941	1632 *
Completeness [%]	86.4	58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG 4000, ammonium formate, sodium bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.18 (mg/ml)
2	1	reservoir	PEG4000	23 (%)
3	1	reservoir	ammonium formate	0.2 (M)
4	1	reservoir	sodium bicine	0.1 (M)	pH9.0