1J0A

Crystal Structure Analysis of the ACC deaminase homologue

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9795, 0.9640
Spacegroup name	P 32 2 1
Unit cell lengths	122.340, 122.340, 115.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.500
Rwork	0.201
R-free	0.26950
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.542
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000 *	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.285
Total number of observations	34161 *
Number of reflections	2668 *	282 *
<I/σ(I)>	7.9	2.9
Completeness [%]	98.6	98.6 *
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	277 *	ammonium sulfate, 2-propanol, phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	sodium potassium phosphate	0.1 (M)	pH6.2-7.0
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	reservoir	2-propanol	3-8 (%(v/v))