1J05
The crystal structure of anti-carcinoembryonic antigen monoclonal antibody T84.66 Fv fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.774, 75.033, 63.105 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.250 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.325 |
| Number of reflections | 91647 | |
| <I/σ(I)> | 6.7 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na, K Phosphate, Na Hepes, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
