1IZQ
F46V mutant of bovine pancreatic ribonuclease A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.700000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.117, 64.117, 63.377 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.24000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fs3 |
RMSD bond length | 0.004 |
RMSD bond angle | 24.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.080 * | 0.428 |
Number of reflections | 23659 * | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 96.3 | 73.4 |
Redundancy | 5 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 25 * | ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
2 | 1 | drop | EDTA | 1 (mM) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | pH6.0 |
4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
5 | 1 | reservoir | 2 (M) |