1IZO
Cytochrome P450 BS beta Complexed with Fatty Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 155.789, 155.789, 111.904 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
Rwork | 0.246 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 21.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.040 | 0.323 |
Total number of observations | 618052 * | |
Number of reflections | 87103 | |
<I/σ(I)> | 16.2 | 1 |
Completeness [%] | 95.1 | 81.5 |
Redundancy | 7.4 * | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 20 * | 10%(w/v) PEG 3350, 50 mM MES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 10 (%(w/v)) | |
3 | 1 | reservoir | MES | 50 (mM) | pH6.8 |