1IZC
Crystal Structure Analysis of Macrophomate synthase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 106.678, 106.678, 120.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.20300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.978 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.081 | 0.315 |
| Number of reflections | 41139 | |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 91.3 | 61 |
| Redundancy | 6.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
