1IZ5
Pyrococcus furiosus PCNA mutant (Met73Leu, Asp143Ala, Asp147Ala): orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.836 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.289, 85.348, 118.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.800 |
| Rwork | 0.273 |
| R-free | 0.29750 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ge8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 0.602 |
| Total number of observations | 214024 * | |
| Number of reflections | 60694 | |
| <I/σ(I)> | 4.7 | 1.4 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.5 | 6.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | ammonium sulfate, 2-methyl-2,4-pentanediol, sodium citrate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 2 | 1 | reservoir | ammonium sulfate | 2.6 (M) | |
| 3 | 1 | reservoir | MPD | 11 (%(v/v)) | |
| 4 | 1 | reservoir | glycerol | 4 (%(v/v)) | |
| 5 | 1 | reservoir | D143A/D147A |
