1IYX
Crystal structure of enolase from Enterococcus hirae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 153.510, 153.510, 90.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.246 |
Rwork | 0.175 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ebh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.093 | 0.342 |
Number of reflections | 30711 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 100.0 * | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 5 * | PEG4000, lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 100 (mM) | pH7.5 |
2 | 1 | reservoir | PEG4000 | 25-30 (%) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | drop | protein | 5-8 (mg/ml) |