1IYD
CRYSTAL STRUCTURE OF ESCHELICHIA COLI BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 154.270, 99.020, 138.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 * - 2.150 |
| Rwork | 0.205 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 * | 0.195 * |
| Total number of observations | 324820 * | |
| Number of reflections | 58165 | |
| Completeness [%] | 99.5 | 96.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 293 | PEG400, HEPES, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | PEG400 | 42 (%(v/v)) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | glutamate | 50 (mM) | |
| 5 | 1 | drop | sodium HEPES | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG400 | 50 (%(v/v)) | |
| 7 | 1 | reservoir | glutarate | 100 (mM) | |
| 8 | 1 | reservoir | glutamate | 50 (mM) |
