1IY2
Crystal structure of the FtsH ATPase domain from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-04 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.647, 101.149, 66.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| Rwork | 0.253 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.125 * | 0.259 * |
| Number of reflections | 4964 | |
| Completeness [%] | 97.1 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | PEG400 | 2.5 (%(v/v)) | |
| 4 | 1 | drop | AMP-PNP | 100 (mM) | |
| 5 | 1 | drop | 40 (mM) |
