1IY1
Crystal structure of the FtsH ATPase domain with ADP from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-06-06 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 73.875, 73.875, 84.611 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 2.800 |
Rwork | 0.207 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.051 * | 0.157 * |
Number of reflections | 6399 | |
Completeness [%] | 98.8 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | Ammonium sulfate, Tris-HCl, ADP, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
2 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
3 | 1 | reservoir | glycerol | 20 (%(v/v)) | |
4 | 1 | drop | AMP-PNP | 100 (mM) | |
5 | 1 | drop | 40 (mM) |