1IXV
Crystal Structure Analysis of homolog of oncoprotein gankyrin, an interactor of Rb and CDK4/6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 278 |
| Detector technology | CCD |
| Collection date | 2002-01-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.433, 61.736, 98.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.300 |
| Rwork | 0.194 |
| R-free | 0.22900 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.460 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.216 |
| Number of reflections | 11582 | |
| <I/σ(I)> | 6.6 | 3.2 |
| Completeness [%] | 99.0 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Adachi, N., (2002) Acta Cryst., D58, 859. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 15-20 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
