1IXV
Crystal Structure Analysis of homolog of oncoprotein gankyrin, an interactor of Rb and CDK4/6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 278 |
Detector technology | CCD |
Collection date | 2002-01-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.433, 61.736, 98.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.300 |
Rwork | 0.194 |
R-free | 0.22900 * |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.460 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.085 | 0.216 |
Number of reflections | 11582 | |
<I/σ(I)> | 6.6 | 3.2 |
Completeness [%] | 99.0 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Adachi, N., (2002) Acta Cryst., D58, 859. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 15-20 (%) | |
3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |