1IXS
Structure of RuvB complexed with RuvA domain III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-13 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.836 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.060, 102.060, 137.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.200 |
Rwork | 0.231 |
R-free | 0.29300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hqc |
RMSD bond length | 0.007 |
RMSD bond angle | 1.334 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.091 | 0.324 |
Total number of observations | 235468 * | |
Number of reflections | 12468 * | |
<I/σ(I)> | 6.8 | 2.1 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RuvA domain III | 0.5 (mM) | |
10 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
2 | 1 | drop | RuvB | 0.5 (mM) | |
3 | 1 | drop | AMPPNP | 1 (mM) | |
4 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
5 | 1 | drop | 0.1 (M) | ||
6 | 1 | drop | glycerol | 10 (%(w/v)) | |
7 | 1 | reservoir | Tris-HCl | 10 (mM) | pH8.0 |
8 | 1 | reservoir | 0.1 (M) | ||
9 | 1 | reservoir | glycerol | 10 (%(w/v)) |