1IXO
Enzyme-analogue substrate complex of Pyridoxine 5'-Phosphate Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 129.800, 155.900, 127.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.26000 |
| Structure solution method | SIRAS |
| Starting model (for MR) | native protein |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.220 |
| Total number of observations | 187532 * | |
| Number of reflections | 57133 * | |
| <I/σ(I)> | 12.2 | 2.7 |
| Completeness [%] | 99.8 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 293 | microseeding, Garrido-Franco, M., (2000) Acta Crystallogr., Sect.D, 56, 1045. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 2 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 10 (%) | |
| 4 | 1 | reservoir | 2 (M) |
