1IXN
Enzyme-Substrate Complex of Pyridoxine 5'-Phosphate Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-06-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 128.900, 156.300, 127.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.300 |
R-factor | 0.19 |
Rwork | 0.180 |
R-free | 0.22400 |
Structure solution method | SIRAS |
Starting model (for MR) | native protein |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.750 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.079 | 0.464 |
Total number of observations | 165177 * | |
Number of reflections | 53504 * | |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 90.4 * | 93.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 * | 293 | microseeding, Garrido-Franco, M., (2000) Acta Crystallogr., Sect.D, 56, 1045. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 2 (mM) | |
3 | 1 | reservoir | PEG6000 | 10 (%) | |
4 | 1 | reservoir | 2 (M) |