1IXL
Crystal structure of uncharacterized protein PH1136 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-04 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9000, 0.9785, 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.500, 57.500, 79.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 1.940 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.117 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.083 | 0.332 * |
| Number of reflections | 11527 | |
| <I/σ(I)> | 8.1 | 3.4 |
| Completeness [%] | 96.8 | 100 |
| Redundancy | 8.23 | 8.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 4 * | Tris-HCl, Ammonium Sulfate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0-9.0 |
| 4 | 1 | reservoir | ammonium sulfate | 2.0-2.8 (M) |
