1IXC

Crystal structure of CbnR, a LysR family transcriptional regulator

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	111
Detector technology	CCD
Collection date	2001-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9796, 0.9798, 0.9721, 0.9862
Spacegroup name	P 21 21 2
Unit cell lengths	71.220, 100.856, 87.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.710 - 2.200
R-factor	0.21907
Rwork	0.218
R-free	0.24527
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.334
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.053	0.235
Number of reflections	32808
<I/σ(I)>	9.4	0.265
Completeness [%]	100.0	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris/HCl, MgSO4, NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris/HCl, MgSO4, NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris/HCl, MgSO4, NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K