1IX6
Aspartate Aminotransferase Active Site Mutant V39F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-12-05 |
Detector | WEISSENBERG |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 156.107, 87.842, 80.507 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
Rwork | 0.205 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 * |
High resolution limit [Å] | 2.200 |
Rmerge | 0.068 * |
Number of reflections | 26929 * |
Completeness [%] | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 10 (mM) | pH7.0 |
3 | 1 | drop | PLP | 0.010 (mM) | |
4 | 1 | drop | 0.3 (mM) | ||
5 | 1 | reservoir | potassium phosphate | 10 (mM) | pH7.0 |
6 | 1 | reservoir | ammonium sulfate | 40 (%sat) |