1IX3
Crystal Structure of Rat Heme Oxygenase-1 in complex with Heme bound to Cyanide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.700, 65.700, 119.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.199 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ivj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 * | 0.284 * |
| Number of reflections | 20629 * | |
| <I/σ(I)> | 8.7 | 2.5 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sugishima, M., (2002) J.Biol.Chem., 277, 45086. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 4 (M) | |
| 2 | 1 | drop | protein | 18 (mg/ml) | |
| 3 | 1 | drop | potassium phosphate | 50 (mM) | pH7.0 |
| 4 | 1 | drop | sodium azide | 5 (mM) |
