1IX2
Crystal Structure of Selenomethionine PcoC, a Copper Resistance Protein from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9793, 0.9643 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 107.650, 107.650, 107.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.081 | 0.302 |
| Number of reflections | 30655 | |
| <I/σ(I)> | 8.5 | 1.8 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | SODIUM CITRATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | SODIUM CITRATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
