1IX1
Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-24 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 68.750, 74.460, 77.180 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 分解能 | 20.000 * - 1.850 |
| R因子 | 0.188 |
| Rwork | 0.185 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 1.920 |
| 分解能 [Å] (高) | 1.850 | 1.850 |
| Total number of observations | 161692 * | |
| 独立反射数 | 57807 | |
| 完全性 [%] | 95.0 | 83.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | Kim, H.W., (2002) Acta Crystallogr., D58, 1874. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 0.010 (mg/ml) | |
| 2 | 1 | reservoir | ADA | 100 (mM) | pH6.5 |
| 3 | 1 | reservoir | lithium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 2 (%(v/v)) | |
| 5 | 1 | reservoir | PEG4000 | 12 (%(w/v)) |
