1IX1

Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-10-24
Detector	MACSCIENCE
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	68.750, 74.460, 77.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.850
R-factor	0.188
Rwork	0.185
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	22.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.920
High resolution limit [Å]	1.850	1.850
Total number of observations	161692 *
Number of reflections	57807
Completeness [%]	95.0	83.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	Kim, H.W., (2002) Acta Crystallogr., D58, 1874. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.010 (mg/ml)
2	1	reservoir	ADA	100 (mM)	pH6.5
3	1	reservoir	lithium sulfate	100 (mM)
4	1	reservoir	2-propanol	2 (%(v/v))
5	1	reservoir	PEG4000	12 (%(w/v))