1IX1
Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-24 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.750, 74.460, 77.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.850 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Total number of observations | 161692 * | |
| Number of reflections | 57807 | |
| Completeness [%] | 95.0 | 83.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | Kim, H.W., (2002) Acta Crystallogr., D58, 1874. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.010 (mg/ml) | |
| 2 | 1 | reservoir | ADA | 100 (mM) | pH6.5 |
| 3 | 1 | reservoir | lithium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 2 (%(v/v)) | |
| 5 | 1 | reservoir | PEG4000 | 12 (%(w/v)) |
