1IWN
Crystal Structure of the Outer Membrane Lipoprotein Receptor LolB Complexed with PEGMME2000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-06-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 71.350, 71.350, 133.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| Rwork | 0.217 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IWM (chain A) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 * | 0.253 * |
| Number of reflections | 10647 | |
| Completeness [%] | 98.2 | 93.4 |
| Redundancy | 19.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | PEGMME2000, sodium acetate, ammonium sulfate, sodium iodide, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG2000MME | 30 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (mM) | pH4.6 |
| 5 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 6 | 1 | reservoir | sodium iodide | 0.15 (M) |
