1IWL

Crystal Structure of the Lipoprotein Localization Factor, LolA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	90
Detector technology	CCD
Collection date	2001-05-16
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	I 2 2 2
Unit cell lengths	55.760, 75.370, 99.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.650
Rwork	0.222
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.043 *	0.236 *
Number of reflections	23909
Completeness [%]	93.2	51.6
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	4 *	PEG8000, sodium cacodilate, zinc acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.4
3	1	reservoir	PEG8000	15 (%(w/v))
4	1	reservoir	sodium cacodylate	80 (mM)	pH6.5
5	1	reservoir	zinc acetate	0.15 (M)
6	1	reservoir	glycerol	20 (%(v/v))