1IWJ
Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Mutant(109K) Cytochrome P450cam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 297 |
Detector technology | CCD |
Collection date | 2002-03-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.160, 104.330, 36.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
Rwork | 0.168 |
R-free | 0.22300 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.054 | 0.054 |
Number of reflections | 27413 | |
<I/σ(I)> | 14 | 12.8 |
Completeness [%] | 95.1 | 79.8 |
Redundancy | 3.2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6 | 293 | ammonium sulfate, pH 6.0, SMALL TUBES, temperature 293K |