1IWI
Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Cytochrome P450cam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 297 |
| Detector technology | CCD |
| Collection date | 2001-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.160, 104.330, 36.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.173 * |
| Rwork | 0.177 |
| R-free | 0.22900 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.134 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.032 | 0.061 |
| Total number of observations | 163126 * | |
| Number of reflections | 27250 | |
| <I/σ(I)> | 13.2 | 11 |
| Completeness [%] | 95.1 | 79.8 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 12-15 * | Poulos, T.L., (1982) J. Biol. Chem., 257, 10427. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 50 (mM) | ||
| 2 | 1 | 1 | 250 (mM) | ||
| 3 | 1 | 1 | dithiothreitol | 50 (mM) | |
| 4 | 1 | 2 | ammonium sulphate | 66-70 (%sat) |
