1IWA

RUBISCO FROM GALDIERIA PARTITA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	201.327, 146.988, 197.233
Unit cell angles	90.00, 99.79, 90.00

Refinement procedure

Resolution	40.000 * - 2.600
R-factor	0.172
Rwork	0.172
R-free	0.23500
RMSD bond length	0.006
RMSD bond angle	1.340 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800 *	40.000
High resolution limit [Å]	2.600 *	2.600
Rmerge	0.103 *	0.306 *
Total number of observations	895084 *
Number of reflections	172258 *
Completeness [%]	98.9 *	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22.7 (mg/ml)
2	1	reservoir	HEPES-NaOH	100 (mM)	pH7.5
3	1	reservoir	PEG4000	12 (%(w/v))
4	1	reservoir	MPD	5 (%(w/v))
5	1	reservoir	glycerol	20 (%(w/v))