1IW9
Crystal Structure of the M Intermediate of Bacteriorhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-18 |
Detector | RIGAKU |
Wavelength(s) | 0.708 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 102.300, 102.300, 112.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.000 * - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.28600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dze |
RMSD bond length | 0.007 |
RMSD bond angle | 1.231 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.068 * | 0.522 * |
Number of reflections | 10066 * | |
Completeness [%] | 92.1 * | 92.1 * |
Redundancy | 5.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 10 * | ammonium sulfate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | purple membrane sheets | 5 (mg/ml) | |
2 | 1 | drop | octylthioglucoside | 2.5 (mg/ml) | |
3 | 1 | drop | ammonium sulfate | 1.0 (M) | |
4 | 1 | drop | 0.16 (M) | ||
5 | 1 | drop | trehalose | 12 (%(w/v)) | |
6 | 1 | drop | 0.04 (%(w/v)) | ||
7 | 1 | drop | sodium citrate | 0.08 (M) | pH5.2 |
8 | 1 | reservoir | ammonium sulfate | 1.9-2.0 (M) |