1IW8
Crystal Structure of a mutant of acid phosphatase from Escherichia blattae (G74D/I153T)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-03 |
Detector | FUJI |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 137.800, 168.200, 58.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.28500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d2t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 * |
Data reduction software | WEIS |
Data scaling software | WEIS |
Phasing software | AMoRE |
Refinement software | CNX (2000.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.084 | |
Number of reflections | 45889 | |
Completeness [%] | 97.5 | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
3 | 1 | reservoir | PEG400 | 38 (%) | |
4 | 1 | reservoir | Tris-HCl | 20 (mM) | pH8.4 |