1IW8
Crystal Structure of a mutant of acid phosphatase from Escherichia blattae (G74D/I153T)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-03 |
| Detector | FUJI |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.800, 168.200, 58.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d2t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 * |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | AMoRE |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | |
| Number of reflections | 45889 | |
| Completeness [%] | 97.5 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG400 | 38 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 20 (mM) | pH8.4 |
