1IW1
Crystal structure of a heme oxygenase (HmuO) from Corynebacterium diphtheriae complexed with heme in the ferrous state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2002-02-23 |
| Detector | RIGAKU JUPITER 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.865, 62.837, 107.208 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.500 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20200 |
| Structure solution method | MR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.710 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 | 0.306 |
| Total number of observations | 651990 * | |
| Number of reflections | 110939 | |
| <I/σ(I)> | 4.8 | 2.2 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 303 | ammonium sulfate, NaI, MES, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | MES | 50 (mM) | pH5.8 |
| 4 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 5 | 1 | reservoir | sodium iodode | 230 (mM) |
