1IVS
CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS VALYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(VAL) AND VALYL-ADENYLATE ANALOGUE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.708 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 411.810, 411.810, 81.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.28100 * |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 22.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 150310 | |
Completeness [%] | 96.5 | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | Fukai, S., (2000) Cell (Cambridge,Mass.), 103, 793. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-10 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.0 (M) | |
4 | 1 | drop | magnesium sulfate | 10 (mM) | |
5 | 1 | drop | 1,8-diaminooctane | 6 (%) | |
6 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
8 | 1 | reservoir | magnesium sulfate | 10 (mM) |