1IV7
Crystal Structure of Single Chain Monellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-19 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 60.650, 36.260, 46.380 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.820 |
| R-factor | 0.20202 |
| Rwork | 0.200 |
| R-free | 0.24084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mol |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.232 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.067 |
| Number of reflections | 17026 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.2 | 277 | PEG8000, potassium phosphate, pH 7.2, micro batch, temperature 277K |
