1IUL
The structure of cell-free ID.343 from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9747, 0.9800, 0.9804, 0.9824 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.098, 64.890, 34.971 |
Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
Resolution | 13.000 - 2.000 |
R-factor | 0.199 |
Rwork | 0.187 |
R-free | 0.27000 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.097 |
Total number of observations | 31394 * | |
Number of reflections | 8037 | |
<I/σ(I)> | 21.8 | |
Completeness [%] | 98.9 | 91.5 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.9 * | 293 * | SODIUM ACETATE, AMMONIUM SULFATE, PEG MME 2000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.2 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.9 |
3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
4 | 1 | reservoir | PEG2000 MME | 15 (%) |