1IUA
Ultra-high resolution structure of HiPIP from Thermochromatium tepidum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.7293 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.697, 58.272, 23.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.800 |
R-factor | 0.0916 |
R-free | 0.11410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 * |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 5.000 * | 0.810 |
High resolution limit [Å] | 0.800 | 0.800 |
Rmerge | 0.052 * | 0.397 * |
Total number of observations | 823255 * | |
Number of reflections | 64373 | 3094 * |
Completeness [%] | 98.6 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 293 * | 0.7M A/S, 0.05M Sodium Citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.2K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH3.5 |