1IUA

Ultra-high resolution structure of HiPIP from Thermochromatium tepidum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.7293
Spacegroup name	P 21 21 21
Unit cell lengths	44.697, 58.272, 23.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 0.800
R-factor	0.0916
R-free	0.11410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	5.000 *	0.810
High resolution limit [Å]	0.800	0.800
Rmerge	0.052 *	0.397 *
Total number of observations	823255 *
Number of reflections	64373	3094 *
Completeness [%]	98.6	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	293 *	0.7M A/S, 0.05M Sodium Citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.2K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)	pH8.0
2	1	reservoir	ammonium sulfate	1.4 (M)
3	1	reservoir	sodium citrate	0.1 (M)	pH3.5