1IU4
Crystal Structure Analysis of the Microbial Transglutaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-02 |
Detector | FUJI |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.410, 117.120, 85.740 |
Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.2026 |
Rwork | 0.199 |
R-free | 0.26600 * |
Structure solution method | MIR |
RMSD bond length | 0.020 |
RMSD bond angle | 3.655 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.040 | 0.130 |
Total number of observations | 187008 * | |
Number of reflections | 55351 | |
<I/σ(I)> | 12 | |
Completeness [%] | 1.0 | 92.1 * |
Redundancy | 3.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | PEG1000, calcium chloride, cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1000 | 25 (%(w/v)) | |
2 | 1 | reservoir | cacodylate-HCl | 100 (mM) | pH5.0 |
3 | 1 | reservoir | 25 (mM) | ||
4 | 1 | drop | protein | 15 (mg/ml) |