1ITX
Catalytic Domain of Chitinase A1 from Bacillus circulans WL-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.50000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.007, 46.839, 55.700 |
| Unit cell angles | 109.29, 95.45, 116.68 |
Refinement procedure
| Resolution | 36.793 - 1.100 |
| R-factor | 0.11735 |
| Rwork | 0.116 |
| R-free | 0.13719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.675 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.793 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.042 | 0.248 |
| Number of reflections | 139823 | |
| <I/σ(I)> | 10.1 | 2.4 |
| Completeness [%] | 98.1 | 97.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | PEG 4000, potassium dihydrophosphate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
