1ITG

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HIV-1 INTEGRASE: SIMILARITY TO OTHER POLYNUCLEOTIDYL TRANSFERASES

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	72.757, 72.757, 66.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.500 *
R-factor	0.202
Rwork	0.202
R-free	0.27900 *
RMSD bond length	0.008
RMSD bond angle	1.507
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.040 *
Total number of observations	52552 *
Number of reflections	6746 *
Completeness [%]	93.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop		0.5 (M)
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	EDTA	1 (mM)
6	1	reservoir	PEG8000	15 (%(w/v))
7	1	reservoir	sodium cacodylate	0.1 (M)
8	1	reservoir	ammonium sulfate	0.2 (M)
9	1	reservoir	dithiothreitol	5 (mM)