1IT9

CRYSTAL STRUCTURE OF AN ANTIGEN-BINDING FRAGMENT FROM A HUMANIZED VERSION OF THE ANTI-HUMAN FAS ANTIBODY HFE7A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	296
Detector technology	DIFFRACTOMETER
Collection date	1999-06-29
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.365, 82.746, 104.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.202
Rwork	0.202
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	30.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.078 *	0.180 *
Total number of observations	61689 *
Number of reflections	11136 *	927 *
<I/σ(I)>	17.1	5.4
Completeness [%]	91.0 *	71.3 *
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	22 *	PEG4000, SODIUM ACETATE, METHANOL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	15 (%)
2	1	reservoir	sodium acetate	0.1 (M)	pH4.6
3	1	reservoir	methanol	30 (%)