1IT8

Crystal structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii complexed with archaeosine precursor, preQ0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-19
Detector	RIGAKU RAXIS V
Wavelength(s)	1.02
Spacegroup name	P 43 21 2
Unit cell lengths	100.467, 100.467, 366.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.500
R-factor	0.2315 *
Rwork	0.232
R-free	0.27850 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iq8
RMSD bond length	0.007
RMSD bond angle	1.370 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.084	0.202
Total number of observations	282730 *
Number of reflections	63242 *	2975 *
<I/σ(I)>	8.69	2.14
Completeness [%]	96.0	92.3
Redundancy	4.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ishitani, R., (2001) Acta Cryst., D57, 1659. *