1IT0

Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2001-05-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	79.540, 95.250, 141.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.000
R-factor	0.191
Rwork	0.191
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xyf
RMSD bond length	0.005
RMSD bond angle	1.260 *
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.370	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.071	0.272
Total number of observations	215954 *
Number of reflections	73487
<I/σ(I)>	12.8	4.9
Completeness [%]	98.4	98
Redundancy	2.88	2.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	298	ammonium sulfate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	25 (%(v/v))	or 27.5%(v/v)
3	1	reservoir	citric acid	0.1 (M)
4	1	reservoir	sodium phosphate	0.2 (M)	pH5.7