1IT0
Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.540, 95.250, 141.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xyf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.260 * |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.370 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.272 |
| Total number of observations | 215954 * | |
| Number of reflections | 73487 | |
| <I/σ(I)> | 12.8 | 4.9 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 2.88 | 2.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | ammonium sulfate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 25 (%(v/v)) | or 27.5%(v/v) |
| 3 | 1 | reservoir | citric acid | 0.1 (M) | |
| 4 | 1 | reservoir | sodium phosphate | 0.2 (M) | pH5.7 |
