1ISQ

Pyrococcus furiosus PCNA complexed with RFCL PIP-box peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	104
Detector technology	DIFFRACTOMETER
Collection date	2001-02-17
Detector	WEISSENBERG
Wavelength(s)	1.0000
Spacegroup name	P 63
Unit cell lengths	91.847, 91.847, 64.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.235
R-free	0.29130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ge8
RMSD bond length	0.007
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.078 *	0.345 *
Total number of observations	60038 *
Number of reflections	13867 *
<I/σ(I)>	19.32	2.24
Completeness [%]	99.9	99.1 *
Redundancy	4.4 *	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	ammonium sulfate, sodium citrate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	1.8 (mM)
2	1	reservoir	sodium citrate	100 (mM)	pH5.5
3	1	reservoir	ammonium sulfate	2.4 (M)
4	1	reservoir	glycerol	10 (%(v/v))