1ISP
Crystal structure of Bacillus subtilis lipase at 1.3A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-07 |
Detector | ADSC QUAMTUM 4r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.200, 58.450, 82.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.300 |
Rwork | 0.192 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.029 |
RMSD bond angle | 2.360 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.068 | 0.315 |
Total number of observations | 169453 * | |
Number of reflections | 44447 | |
<I/σ(I)> | 6.5 | 2.2 |
Completeness [%] | 96.5 | 79.9 |
Redundancy | 3.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | PEG 8000, glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.7-8.8 |
2 | 1 | reservoir | PEG8000 | 6-9 (%(w/v)) | |
3 | 1 | reservoir | glycerol | 17 (%(v/v)) | |
4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
5 | 1 | drop | protein | 15 (mg/ml) |