1ISP
Crystal structure of Bacillus subtilis lipase at 1.3A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-07 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.200, 58.450, 82.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| Rwork | 0.192 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.360 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.068 | 0.315 |
| Total number of observations | 169453 * | |
| Number of reflections | 44447 | |
| <I/σ(I)> | 6.5 | 2.2 |
| Completeness [%] | 96.5 | 79.9 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | PEG 8000, glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.7-8.8 |
| 2 | 1 | reservoir | PEG8000 | 6-9 (%(w/v)) | |
| 3 | 1 | reservoir | glycerol | 17 (%(v/v)) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 5 | 1 | drop | protein | 15 (mg/ml) |
