1ISN
Crystal structure of merlin FERM domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.408, 67.408, 179.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DPS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.069 | 0.316 |
| Total number of observations | 32050 * | |
| Number of reflections | 8807 | |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 90.5 | 75.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 4 * | PEG6000, LiCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13.6 (mg/ml) | |
| 2 | 1 | drop | MES | 10 (mM) | pH6.8 |
| 3 | 1 | drop | PEG6000 | 7.5 (%) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | 250 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 7 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
| 8 | 1 | reservoir | PEG6000 | 15 (%) | |
| 9 | 1 | reservoir | 500 (mM) |
