1ISJ
Crystal Structure Analysis of BST-1/CD157 complexed with NMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-18 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 0.836 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.804, 112.590, 129.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.300 |
| Rwork | 0.215 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.241 |
| Number of reflections | 38880 | |
| <I/σ(I)> | 15.3 | 33.6 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 27.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | ammmonium sulfate, citrate, NMN, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
