1ISH
Crystal Structure Analysis of BST-1/CD157 complexed with ethenoNADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.836 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.165, 112.458, 131.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.205 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.203 |
| Number of reflections | 33495 | |
| <I/σ(I)> | 3.83 | 17.54 |
| Completeness [%] | 96.3 | 91.3 |
| Redundancy | 30.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 | 20 * | ammonium sulfate, citrate, ethenoNADP, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | NMN | 62.5 (mM) | |
| 2 | 1 | 1 | ATP gammaS | 37.5 (mM) | |
| 3 | 1 | 1 | ethenoNADP | 62.5 (mM) | |
| 4 | 1 | 1 | ethenoNAD | 62.5 (mM) |
