1ISE
Crystal structure of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 2000-07-24 |
Detector | WEISSENBERG |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.024, 49.267, 49.365 |
Unit cell angles | 90.00, 110.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
Rwork | 0.239 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.704 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 10611 | |
Completeness [%] | 98.4 | 93.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 50mM MES-NaOH, PEG 1500, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |